Aflatoxin M1 ELISA Test Kit
Product Description
REAGEN™ Aflatoxin M1 ELISA Test Kit is a competitive enzyme
immunoassay for the quantitative analysis of Aflatoxin M1 in
cereals, meat/fish, feed, peanuts, pistachios cheese,milk powder
and milk .
The unique features of the kit are:
1.High recovery (75-120%),rapid(10-15minutes), cost-effective
extraction methods.
2.High sensitivity (0.02 ng/g or ppb) .
3.High reproducibility.
4.A quick ELISA assay (less than 1.5 hours regardless of number of
samples).
5.Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
toxin of interest has been coated in plate wells. During the
analysis, sample is added along with the primary antibody specific
for target toxin. If the target is present in the sample, it will
compete for the antibody, thereby preventing the antibody from
binding to the toxin attached to the well. The second antibody,
tagged with a peroxidase enzyme, targets the primary antibody that
is complexed to the toxin coated on the wells. The resulting color
intensity, after addition of substrate, has an inverse relationship
with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Aflatoxin M1 ELISA Test Kit has the Aflatoxin M1acity for
96 determinations or testing of 42 samples in duplicate (assuming
12 wells for standards). Return any unused microwells to the foil
bag and reseal them with the desiccant provided in the original
package. Store the kit at 2-8°C *. The shelf life is 12 months when
the kit is properly stored.
| Kit Contents | Amount | Storage |
| Aflatoxin M1 Antibody-Coated Plate | 1 x 96-well Plate(8 wells x 12 strips) | 2-8°C |
Aflatoxin M1 Standards: Negative control (white cap tube) 0.02ng/mL (yellow cap tube) 0.06ng/mL (orange cap tube) 0.18 ng/mL (pink cap tube) 0.54ng/mL (purple cap tube) 1.62ng/mL (blue cap tube) 10 ng/mL (spiking, red cap tube) |
0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL |
2-8°C
2-8°C
|
| Aflatoxin M1 Antibody #1 | 6ml | 2-8°C
|
| HRP-Conjugated Antibody #2 | 11 mL |
| 20X Wash Solution ** | 25 mL | |
| Stop Buffer ** | 11 mL |
| TMB Substrate ** | 11 mL |
| Milk Ext.Buffer I | 28 mLx3 |
| 10x PBS | 10 mL |
| Ext.Buffer A (optional) | 1.5 mL |
| Ext.Buffer B (optional) | 1.5 mL |
| Ext.Buffer C (optional) | 1.5 mL |
* If you are not planning to use the kit for over 3 months, store
Aflatoxin M1 Antibody #1 and 1X HRP-Conjugated Antibody #2 at -20°C
or in a freezer.
Sensitivity
| Sample type | Detection Limit (ng/g or ppb) |
| Fresh milk | 0.03 |
| Milk powder | 0.1 |
| Yogurt | 0.05 |
| Whey powder | 0.1 |
| Condensed milk | 0.15 |
Specificity
Analytes | Cross-Reactivity (%) |
Aflatoxin M1 | 100.0 |
Aflatoxin B1 | 36 |
Aflatoxin B2 | 25 |
Aflatoxin M2 | 19 |
Aflatoxin G1 | 17 |
Aflatoxin G2 | 3 |
