Dioxin ELISA Test Kit

Product Description

REAGEN™ Dioxin/Furan ELISA is an indirect immunoassay (ELISA) for the quantitative and sensitive detection of Dioxins and Furans in water, soil,Fish/shrimp/meat samples. If necessary, positive samples can be confirmed by instrumental analysis or other conventional methods.

The unique features of the kit are:

1. a high recovery rate of 80 - 105%.

2. A quick ELISA assay (less than 2 hours regardless of number of samples).

3. High reproducibility.

Procedure Overview

The test is an indirect competitive ELISA that allows the sensitive detection of Dioxins and Furans.It is based on the recognition of Dioxins and Furans congeners by specific antibodies. Dioxins and Furans when present in a sample and a Dioxin/Furan-protein analogue immobilized on the plate compete for the binding sites of antibodies in solution. After a washing step, a second antibody-HRP label is added. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of the Dioxin/Furan present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader.

Kit Contents, Storage and Shelf Life

REAGEN™Dioxin/Furan ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.