Cimaterol ELISA Test Kit
Product Description
REAGEN™Cimaterol ELISA Test Kit is a competitive enzyme immunoassay for
the quantitative analysis of cimaterol in feed, meat/tissue, milk
and urine. Cimaterol can improve the meat/fat ratio in fattened
animals and accelerate livestock growth. They also have a relaxing
effect on non-striated musculature and can be used as antiasthmatic
and tocolytic agents in human beings. Therefore, excess cimaterol
residues in livestock products may lead to health risks for
consumers. This has led to a prohibition of cimaterol use in food
production. The amount of cimaterol residues can be determined by
methods such as HPLC or GC-MS. However, these traditional methods
usually require very expensive preparation procedures.
Alternatively, enzyme immunoassay (ELISA) can be used as a
screening system, which is simple, rapid, sensitive and
cost-effective compared with traditional methods.
The unique features of the kit are:
1. Rapid (10 - 30 minutes) extraction method for various samples
with high recovery (75 - 95%).
2. High sensitivity (0.25 ng/g or ppb) and low detection limit
(0.125 ng/g or ppb for meat/Chicken Liver, 1.25 ng/g or ppb for
urine, and 2.5 ng/g or ppb for feed).
3. High reproducibility.
4. A quick ELISA assay (less than 2 hours regardless of number of
samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
drug of interest has been coated in the plate wells. During the
analysis, sample is added along with the primary antibody specific
for the target drug. If the target is present in the sample, it
will compete for the antibody, thereby preventing the antibody from
binding to the drug attached to the well. The secondary antibody,
tagged with a peroxidase enzyme, targets the primary antibody that
is complexed to the drug coated on the plate wells. The resulting
color intensity, after addition of substrate, has an inverse
relationship with the target concentration in the sample.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 2.5 |
| Meat/Chicken Liver | 0.125 (Method I); 0.5 (Method II) |
| Swine/Bovine Liver and Kidney | 0.5 |
| Milk | 1.25 |
| Urine/Serum | 1.25 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Cimaterol | 100 |
| Clenbuterol | 69 |
| Mapenterol | 15 |
| Tolubuterol | 12 |
| Terbutaline | 2.3 |
| Mabuterol | 0.5 |
| Pirbuterol | <0.1 |
| Isoprenaline | <0.1 |
