Doxycycline ELISA Test Kit
Product Description
REAGEN™ Doxycycline ELISA Test Kit is a competitive enzyme immunoassay for
the quantitative analysis of doxycycline in egg, honey, meat, fish
, shrimp and milk.
The unique features of the kit are:
1.High recovery (75-120%), rapid and cost-effective extraction
methods.
2.High sensitivity (0.1ng/g or ppb) and low detection limit for
samples.
3.High reproducibility.
4.A quick ELISA assay (less than 1 hours regardless of number of
samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
doxycycline-BSA has been coated in the plate wells. During the
analysis, sample and the doxycycline antibody are added along with
secondary antibody, tagged with a peroxidase enzyme. If the
doxycycline residue is present in the sample, it will compete for
the doxycycline antibody, thereby preventing the antibody from
binding to the doxycycline-BSA attached to the well. The resulting
color intensity, after addition of the HRP substrate (TMB), has an
inverse relationship with the doxycycline residue concentration in
the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Doxycycline ELISA Test Kit has the capacity for 96 determinations
or testing of 42 samples in duplicate (assuming 12 wells for
standards). Return any unused micro-wells to the foil bag and
reseal them with the desiccant provided in the original package.
Store the kit at 2-8°C . The shelf life is 12 months when the kit
is properly stored.
| Kit Contents | Amount | Storage |
| Doxycycline-Coated Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8℃ |
Doxycycline Standards: Negative control (white Cap tube) 0.1ng/mL (yellow Cap tube) 0.3 ng/mL (orange Cap tube) 0.9 ng/mL (pink Cap tube) 2.7 ng/mL (purple Cap tube) 8.1 ng/mL (blue Cap tube) 500ng/mL (Spiking, red Cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0mL 1.0 mL 1.0 mL 1.0 mL |
2-8℃
|
| Doxycycline Antibody #1 | 6 mL | 2-8℃ |
| HRP-Conjugated Ab #2 | 6 mL |
| 20X Wash Solution | 30 mL |
| 10X Sample Ext. Buffer | 30 mL |
| Stop Buffer | 12 mL |
| TMB Substrate | 12 mL |
If you are not planning to use the kit for over 1 month, storing
Doxycycline Standard Stock, Doxycycline Antibody #1 and
HRP-Conjugated Ab #2 at －20℃ or in a freezer is recommended.
Sensitivity
| Sample Type | Detection Limit (ng/g or ppb) |
| Egg | 1.5 |
| Honey/Milk | 1 |
| Meat/Fish/Shrimp | 1 |
Specificity
| Analytes | Cross-Reactivity (%) |
| Doxycycline | 100 |
| Chlortetracycline | 24 |
| Oxytetracycline | 21 |
| Tetracycline | 15 |
