Ofloxacin ELISA Test Kit

Product Description

REAGEN™Ofloxacin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect ofloxacin to as low as 0.1 ppb level in various sample types and to satisfy customer concerns about food safety.

The unique features of the kit

1. The extraction of ofloxacin from meat/tissue with a high recovery rate.

2. A quick ELISA assay (less than 1 hours regardless of number of samples).

3. High reproducibility Procedure Overview.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and HRP-Conjugated Antibody #2 are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Sensitivity (Detection Limit)

Specificity (Cross-Reactivity)