Gentamicin ELISA Test Kit
Product Description
REAGEN™ Gentamicin ELISA Test Kit is a competitive enzyme immunoassay for
the quantitative analysis of Gentamicin in egg, honey, feed,
tissue( liver/meat/chicken/fish), urine,serum,milk and milk powder.
The unique features of the kit are:
1. High recovery in a variety of samples, rapid , and
cost-effective extraction methods.
2. High sensitivity (0.02 ng/g or ppb)
3. High reproducibility.
4. A quick ELISA assay (less than 1 hours regardless of number of
samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
drug of interest has been coated in the plate wells. During the
analysis, sample is added along with the primary antibody specific
for the target drug. If the target is present in the sample, it
will compete for the antibody, thereby preventing the antibody from
binding to the drug attached to the well. The secondary antibody,
tagged with a peroxidase enzyme, targets the primary antibody that
is complexed to the drug coated on the plate wells. The resulting
color intensity, after addition of substrate, has an inverse
relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Gentamicin ELISA Test Kit has the capacity for 96 determinations or
testing of 42 samples in duplicate (assuming 12 wells for
standards). Return any unused microwells to the foil bag and reseal
them with the desiccant provided in the original package. Store the
kit at 2-8°C *. The shelf life is 12 months when the kit is
properly stored.
| Kit Contents | Amount | Storage |
| Gentamicin-coated Plate | 1x96-well Plate(8wells x 12 strips) | 2-8°C |
Gentamicin Standards: Negative control (white cap tube) 0.02 ng/mL (yellow cap tube) 0.06ng/mL (orange cap tube) 0.2 ng/mL (pink cap tube) 0.6ng/mL (purple cap tube) 1.5ng/mL (blue cap tube) 50 ng/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL | 2-8°C |
| Gentamicin Antibody #1 | 6 mL | 2-8°C |
| HRP-Conjugated Antibody #2 | 6.0 mL | 2-8°C |
| 10X Sample Extraction Buffer | 30 mL | 2-8°C |
| 10X Sample Dilution Buffer | 30 mL | 2-8°C |
| 20X Wash Solution | 30 mL | 2-8°C |
| Stop Buffer | 12 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
| 10X Gentamicin Extraction Buffer (Optional) | 30 mL | 2-8°C |
| Gentamicin Balance Buffer (Optional) | 3.0 mL | 2-8°C |
* If you are not planning to use the kit for over 1 months, store
Gentamicin Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or
in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Egg | 0.2 |
| Feed | 0.5 |
| Honey | 0.2 |
| Tissue(liver/meat/chicken/fish) | 0.2 |
| Milk | 0.02 |
| Milk Powder | 0.2 |
| Urine/Serum | 0.4 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Gentamicin | 100 |
